Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xxvii
... Expression Libraries in Plasmid and Bacteriophage λ Vectors 12.4 The Relative Advantages of Plasmid and Bacteriophage λ Expression Vectors 12.4 Genomic DNA and cDNA Expression Libraries 12.6 Expression Libraries Constructed in Plasmids ...
... Expression Libraries in Plasmid and Bacteriophage λ Vectors 12.4 The Relative Advantages of Plasmid and Bacteriophage λ Expression Vectors 12.4 Genomic DNA and cDNA Expression Libraries 12.6 Expression Libraries Constructed in Plasmids ...
Page xxxiii
... Expression in Mammalian Cells 16.68 Cloning by Expression of cDNA Clones 16.69 Expression Cloning of Genomic DNA 16.70 References 16.73 17 Expression of Cloned Genes in Escherichia coli Production of Fusion Proteins 17.3 Vector Systems ...
... Expression in Mammalian Cells 16.68 Cloning by Expression of cDNA Clones 16.69 Expression Cloning of Genomic DNA 16.70 References 16.73 17 Expression of Cloned Genes in Escherichia coli Production of Fusion Proteins 17.3 Vector Systems ...
Page 1-36
... expression of prokaryotic genes in E. coli , 5.58 , 17.11-17.16 , 17.31- 17.33 in mammalian expression vectors , 16.5-16.6 . See also Mammalian cells , expression vectors for synthesis of RNA probes . See Bacteriophage - encoded DNA ...
... expression of prokaryotic genes in E. coli , 5.58 , 17.11-17.16 , 17.31- 17.33 in mammalian expression vectors , 16.5-16.6 . See also Mammalian cells , expression vectors for synthesis of RNA probes . See Bacteriophage - encoded DNA ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml