Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-95
... extracts prepared from bacteria infected with bacteriophage A mutant in genes required for assembly of bacteriophage ... extracts have been prepared from cells infected with A and E or D and E strains ; alternatively , extracts prepared ...
... extracts prepared from bacteria infected with bacteriophage A mutant in genes required for assembly of bacteriophage ... extracts have been prepared from cells infected with A and E or D and E strains ; alternatively , extracts prepared ...
Page 2-98
... extracts . The methods given below will be of use primarily to workers who use packaging extracts in such large quantity that it would be improvident to purchase them . Packaging extracts are usually prepared by growing the appropriate ...
... extracts . The methods given below will be of use primarily to workers who use packaging extracts in such large quantity that it would be improvident to purchase them . Packaging extracts are usually prepared by growing the appropriate ...
Page 2-104
... extract . 2. Mix gently . When the combined extracts are almost totally thawed , add the DNA to be packaged ( up to 1 μg dissolved in 5 μl of 10 mM Tris . Cl [ pH 8.0 ] , 10 mM MgCl2 ) . Mix with a fine glass stirring rod ( e.g. , a ...
... extract . 2. Mix gently . When the combined extracts are almost totally thawed , add the DNA to be packaged ( up to 1 μg dissolved in 5 μl of 10 mM Tris . Cl [ pH 8.0 ] , 10 mM MgCl2 ) . Mix with a fine glass stirring rod ( e.g. , a ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml