Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 56
Page 1-78
... Final concentration 10 mM 10 ml 45 mM 8.91 g 10 mм 1.47 g 100 mм 7.46 g 3 mM 0.80 g 1 M MES ( 2- [ N - morpholino ] ethanesulfonic acid ; Ferguson et al . 1980 ) is prepared by dissolving 19.52 g of MES ( Good and Izawa 1972 ) in 80 ml ...
... Final concentration 10 mM 10 ml 45 mM 8.91 g 10 mм 1.47 g 100 mм 7.46 g 3 mM 0.80 g 1 M MES ( 2- [ N - morpholino ] ethanesulfonic acid ; Ferguson et al . 1980 ) is prepared by dissolving 19.52 g of MES ( Good and Izawa 1972 ) in 80 ml ...
Page 2-80
... final concentration of 20 mм . 5. Add pronase ( self - digested ) to a final concentration of 0.5 mg / ml , or add proteinase K to a final concentration of 50 μg / ml . Self - digested pronase is prepared by dissolving powdered pronase ...
... final concentration of 20 mм . 5. Add pronase ( self - digested ) to a final concentration of 0.5 mg / ml , or add proteinase K to a final concentration of 50 μg / ml . Self - digested pronase is prepared by dissolving powdered pronase ...
Page 7-14
... final concentrations of 10 mm and 0.1 mм , respectively , and then add placental RNAase inhibitor or vanadyl- ribonucleoside complexes to a final concentration of 1000 units / ml or 10 mм , respectively . 12. Add RNAase - free ...
... final concentrations of 10 mm and 0.1 mм , respectively , and then add placental RNAase inhibitor or vanadyl- ribonucleoside complexes to a final concentration of 1000 units / ml or 10 mм , respectively . 12. Add RNAase - free ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml