Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 153
The plasmid DNA is cleaved with a restriction enzyme and joined in vitro to
foreign DNA . The resulting recombinant plasmids are then used to transform
bacteria . In practice , however , the plasmid vector must be carefully chosen to
minimize ...
The plasmid DNA is cleaved with a restriction enzyme and joined in vitro to
foreign DNA . The resulting recombinant plasmids are then used to transform
bacteria . In practice , however , the plasmid vector must be carefully chosen to
minimize ...
Page 154
TABLE 1.2 Ligation of Fragments of Foreign DNA to Plasmid Vectors Termini
carried by Requirements for fragment of foreign DNA cloning Comments Blunt -
ended high concentrations of background of nonrecombinant DNAs and ligase ...
TABLE 1.2 Ligation of Fragments of Foreign DNA to Plasmid Vectors Termini
carried by Requirements for fragment of foreign DNA cloning Comments Blunt -
ended high concentrations of background of nonrecombinant DNAs and ligase ...
Page 159
ended molecules therefore require much higher concentrations both of
bacteriophage T4 DNA ligase and of foreign and plasmid DNAs . In addition , the
efficiency of this type of reaction is often increased by addition of low
concentrations of ...
ended molecules therefore require much higher concentrations both of
bacteriophage T4 DNA ligase and of foreign and plasmid DNAs . In addition , the
efficiency of this type of reaction is often increased by addition of low
concentrations of ...
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User Review - iayork - LibraryThingthe BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review
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Common terms and phrases
activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies completely concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome growth Hindi host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations nucleic acids obtained original packaging particles pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes problem procedure promoter protein purified reaction recombinant Recover region remove replication restriction enzyme room temperature Sall segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume Xbal yield
References to this book
Bibliographic information
| Title | Molecular Cloning: A Laboratory Manual, Volume 1 Molecular Cloning: A Laboratory Manual, Joseph Sambrook |
| Authors | Joseph Sambrook, E. F. Fritsch, Tom Maniatis |
| Edition | 2 |
| Publisher | Cold Spring Harbor Laboratory, 1989 |
| Original from | the University of Michigan |
| Digitized | 23 Jul 2008 |
| ISBN | 0879693096, 9780879693091 |
| Length | 676 pages |
| Export Citation | BiBTeX EndNote RefMan |