Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-53
... foreign DNA and vector molecules that have recircularized without insertion of foreign DNA . Recircularization of the vector can be limited to some extent by adjusting the concentrations of the foreign DNA and vector DNA in the ligation ...
... foreign DNA and vector molecules that have recircularized without insertion of foreign DNA . Recircularization of the vector can be limited to some extent by adjusting the concentrations of the foreign DNA and vector DNA in the ligation ...
Page 1-54
... foreign DNAs may be eliminated recombinant plasmids may carry tandem copies of foreign DNA restriction sites at junctions between plasmid and foreign DNAs are usually conserved background of nonrecombinant clones is low foreign DNA is ...
... foreign DNAs may be eliminated recombinant plasmids may carry tandem copies of foreign DNA restriction sites at junctions between plasmid and foreign DNAs are usually conserved background of nonrecombinant clones is low foreign DNA is ...
Page 1-59
... Foreign DNA The variety of restriction sites in plasmid vectors is now extremely large , and it is often possible to find a vector that carries exactly the same restriction sites as the fragment of foreign DNA itself . This has the ...
... Foreign DNA The variety of restriction sites in plasmid vectors is now extremely large , and it is often possible to find a vector that carries exactly the same restriction sites as the fragment of foreign DNA itself . This has the ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml