Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 6-7
... gel melts and the DNA denatures . Several different buffers are available for electrophoresis of native double- stranded DNA . These contain EDTA ( pH ... Gel Electrophoresis Because agarose gel electrophoresis Gel Electrophoresis of DNA 6.7.
... gel melts and the DNA denatures . Several different buffers are available for electrophoresis of native double- stranded DNA . These contain EDTA ( pH ... Gel Electrophoresis Because agarose gel electrophoresis Gel Electrophoresis of DNA 6.7.
Page 6-8
... Gel Electrophoresis Because agarose gel electrophoresis is both forgiving and adaptable , many successful configurations and sizes of electrophoresis tanks have been de- signed during the last 15 years . The choice among these different ...
... Gel Electrophoresis Because agarose gel electrophoresis is both forgiving and adaptable , many successful configurations and sizes of electrophoresis tanks have been de- signed during the last 15 years . The choice among these different ...
Page 1-16
... gel electrophoresis ( FIGE ) . See Pulsed - field gel electrophoresis FIGE ( field - inversion gel electrophoresis ) . See Pulsed - field gel electrophoresis Filamentous bacteriophages . See also M13 bacteriophage ; Phagemids as cloning ...
... gel electrophoresis ( FIGE ) . See Pulsed - field gel electrophoresis FIGE ( field - inversion gel electrophoresis ) . See Pulsed - field gel electrophoresis Filamentous bacteriophages . See also M13 bacteriophage ; Phagemids as cloning ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml