Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-108
... Genetic transforma- tion of Campylobacter jejuni with plas- mid DNA . Proc . Natl . Acad . Sci . 85 : 856 . Minton , N.P. , S.P. Chambers , S.E. Prior , S.T. Cole , and T. Garnier . 1988. Copy number and mobilization properties of PUC ...
... Genetic transforma- tion of Campylobacter jejuni with plas- mid DNA . Proc . Natl . Acad . Sci . 85 : 856 . Minton , N.P. , S.P. Chambers , S.E. Prior , S.T. Cole , and T. Garnier . 1988. Copy number and mobilization properties of PUC ...
Page 2-6
... genetic map of wild - type bacteriophage A. The general locations of genes that encode various lytic and lysogenic functions are indicated at the top of the figure by brackets . The genetic map below the brackets shows the specific ...
... genetic map of wild - type bacteriophage A. The general locations of genes that encode various lytic and lysogenic functions are indicated at the top of the figure by brackets . The genetic map below the brackets shows the specific ...
Page 1-21
... genetic marker in E. coli host strain , 2.57 , 4.12 , 17.13 marker in XORF8 , 2.51 lac operon effect of lacla mutation , 4.12 A ( lac - proAB ) , deletion of lac operon , 4.12 A ( lac - proAB ) , deletion of lac operon , ge- netic ...
... genetic marker in E. coli host strain , 2.57 , 4.12 , 17.13 marker in XORF8 , 2.51 lac operon effect of lacla mutation , 4.12 A ( lac - proAB ) , deletion of lac operon , 4.12 A ( lac - proAB ) , deletion of lac operon , ge- netic ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml