Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 74
Page 2-11
... length , from PR through the cosR site ) is necessary for lytic growth of the bacteriophage ; the central one third ... lengths of their genomes are greater than 105 % or less than 78 % of that of wild - type bacteriophage λ . It is ...
... length , from PR through the cosR site ) is necessary for lytic growth of the bacteriophage ; the central one third ... lengths of their genomes are greater than 105 % or less than 78 % of that of wild - type bacteriophage λ . It is ...
Page 2-25
... length . Charon 33 can accept HindIII fragments up to 19 kb in length and SacI inserts up to 10 kb . The deletion also allows the vector to be used as an EcoRI - SalI vector that retains gam in the recombinants , which allows them to ...
... length . Charon 33 can accept HindIII fragments up to 19 kb in length and SacI inserts up to 10 kb . The deletion also allows the vector to be used as an EcoRI - SalI vector that retains gam in the recombinants , which allows them to ...
Page 7-79
... length of the resulting end - labeled cDNA , as measured by electrophoresis through a polyacrylamide gel under denaturing conditions , reflects the distance between the end - labeled nu- cleotide of the primer and the 5 ' terminus of ...
... length of the resulting end - labeled cDNA , as measured by electrophoresis through a polyacrylamide gel under denaturing conditions , reflects the distance between the end - labeled nu- cleotide of the primer and the 5 ' terminus of ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
Copyright | |
96 other sections not shown
Other editions - View all
Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml