Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-62
... reaction that receives no DNA at all • A transformation reaction that receives a known amount of a standard preparation of closed circular plasmid DNA Most of the time , transformations ... Ligations and Transformations 1 LIGATION REACTIONS.
... reaction that receives no DNA at all • A transformation reaction that receives a known amount of a standard preparation of closed circular plasmid DNA Most of the time , transformations ... Ligations and Transformations 1 LIGATION REACTIONS.
Page 1-63
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. LIGATION REACTIONS Setting Up Ligation Reactions ... reaction conditions . Ligation of one end of DNA to another can be regarded as a bimolecular reaction whose velocity ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. LIGATION REACTIONS Setting Up Ligation Reactions ... reaction conditions . Ligation of one end of DNA to another can be regarded as a bimolecular reaction whose velocity ...
Page 2-94
... ligation reaction are perfect . Since this is rarely the case , it is advisable to carry out pilot reactions to ... ligation mixture should be as small as possible ( 10 μl or less ) . It is essential to include controls that contain ...
... ligation reaction are perfect . Since this is rarely the case , it is advisable to carry out pilot reactions to ... ligation mixture should be as small as possible ( 10 μl or less ) . It is essential to include controls that contain ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml