Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 46
Page 1-23
... linear and closed circular DNA molecules . Ethidium bromide binds to DNA by intercalating between the bases , causing the double helix to unwind . This leads to an increase in the length of linear DNA molecules and to the introduction ...
... linear and closed circular DNA molecules . Ethidium bromide binds to DNA by intercalating between the bases , causing the double helix to unwind . This leads to an increase in the length of linear DNA molecules and to the introduction ...
Page 1-56
... linear molecules , adjust the salt concentration appropriately and add the second enzyme . At the same time , set up a separate pilot reaction that contains circular plasmid DNA and only the second of the two restriction enzymes . When ...
... linear molecules , adjust the salt concentration appropriately and add the second enzyme . At the same time , set up a separate pilot reaction that contains circular plasmid DNA and only the second of the two restriction enzymes . When ...
Page 2-4
... linear genomes COS COS cleavage by A protein COS encapsidation FIGURE 2.1 A schematic diagram of the bacteriophage A life cycle . Double - stranded bacteriophage λ DNA , which enters the bacterial host as a linear duplex , is indicated ...
... linear genomes COS COS cleavage by A protein COS encapsidation FIGURE 2.1 A schematic diagram of the bacteriophage A life cycle . Double - stranded bacteriophage λ DNA , which enters the bacterial host as a linear duplex , is indicated ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml