Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-8
... lysogenic pathways depends on an intricate balance between the activities of a number of host and bacteriophage gene products in the infected cell . To establish lysogeny , proteins encoded by genes cII and cIII ( whose transcriptions ...
... lysogenic pathways depends on an intricate balance between the activities of a number of host and bacteriophage gene products in the infected cell . To establish lysogeny , proteins encoded by genes cII and cIII ( whose transcriptions ...
Page 2-95
... lysogens of the appropriate genotype ( amber mutations in the A , D , or E gene ) . The lysogens also carry one or more of ... lysogenic state when the host bacteria are grown at 32 ° C ; lytic growth of the bacteriophage is induced by ...
... lysogens of the appropriate genotype ( amber mutations in the A , D , or E gene ) . The lysogens also carry one or more of ... lysogenic state when the host bacteria are grown at 32 ° C ; lytic growth of the bacteriophage is induced by ...
Page 2-98
... lysogenic strain is used , and only one extract is prepared . ( 2 ) The lysogenic bacteria are a strain of E. coli C. A single lysogen can be used to prepare packaging extracts for two reasons . First , the bacteriophage A genome lysogenic ...
... lysogenic strain is used , and only one extract is prepared . ( 2 ) The lysogenic bacteria are a strain of E. coli C. A single lysogen can be used to prepare packaging extracts for two reasons . First , the bacteriophage A genome lysogenic ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml