Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 54
Page xxxvi
... Medium ( Luria - Bertani Medium ) A.1 NZCYM Medium A.1 NZYM Medium A.1 NZM Medium A.1 Terrific Broth A.2 SOB Medium A.2 SOC Medium A.2 2 × YT Medium A.3 M9 Minimal Medium A.3 MEDIA CONTAINING AGAR OR AGAROSE A.4 STORAGE MEDIA A.5 Stab ...
... Medium ( Luria - Bertani Medium ) A.1 NZCYM Medium A.1 NZYM Medium A.1 NZM Medium A.1 Terrific Broth A.2 SOB Medium A.2 SOC Medium A.2 2 × YT Medium A.3 M9 Minimal Medium A.3 MEDIA CONTAINING AGAR OR AGAROSE A.4 STORAGE MEDIA A.5 Stab ...
Page 4-23
... medium are unlikely to contain a significant number of female cells . In contrast , plaques formed on richer medium ( 2 × YT or LB ) may contain a proportion of auxotrophic female cells that are resistant to infection by bacteriophage ...
... medium are unlikely to contain a significant number of female cells . In contrast , plaques formed on richer medium ( 2 × YT or LB ) may contain a proportion of auxotrophic female cells that are resistant to infection by bacteriophage ...
Page 4-24
... medium into a sterile tube ( 13 mm × 100 mm ) . 2. Touch the surface of the chosen plaque with the end of a sterile , 8 - cm - long wooden stick ( sometimes called applicator or orange stick ) . Immediately immerse the end of the stick ...
... medium into a sterile tube ( 13 mm × 100 mm ) . 2. Touch the surface of the chosen plaque with the end of a sterile , 8 - cm - long wooden stick ( sometimes called applicator or orange stick ) . Immediately immerse the end of the stick ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml