Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-23
... method can therefore be used with any strain of E. coli grown in cultures of any size . It is curious , however , that many laboratories use one method to process minipreps and another method to isolate plasmid DNA from large - scale ...
... method can therefore be used with any strain of E. coli grown in cultures of any size . It is curious , however , that many laboratories use one method to process minipreps and another method to isolate plasmid DNA from large - scale ...
Page 2-118
... method ( E.F. Fritsch , unpubl . ) and the liquid culture method ( Leder et al . 1977 ) . Both methods work equally well , although there are occasional problems with the yield and purity of the bacteriophage DNA . If trouble should ...
... method ( E.F. Fritsch , unpubl . ) and the liquid culture method ( Leder et al . 1977 ) . Both methods work equally well , although there are occasional problems with the yield and purity of the bacteriophage DNA . If trouble should ...
Page 1-2
... method , 16.37 cells growing in suspension , method , 16.38 modified ( Chen and Okayama ) method , 16.39-16.40 Calf intestinal alkaline phosphatase . See CIP Capillary transfer of nucleic acids from agarose gels to solid supports . See ...
... method , 16.37 cells growing in suspension , method , 16.38 modified ( Chen and Okayama ) method , 16.39-16.40 Calf intestinal alkaline phosphatase . See CIP Capillary transfer of nucleic acids from agarose gels to solid supports . See ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml