Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 40
Page 1-40
... tube , and add 3 ml of an ice - cold solution of 5 м LiCl . Mix well , and then centrifuge the solution at 10,000 ... microfuge tube and store at room temperature for 30 minutes . 5. Add 500 μl of 1.6 м NaCl containing 13 % ( w / v ) ...
... tube , and add 3 ml of an ice - cold solution of 5 м LiCl . Mix well , and then centrifuge the solution at 10,000 ... microfuge tube and store at room temperature for 30 minutes . 5. Add 500 μl of 1.6 м NaCl containing 13 % ( w / v ) ...
Page 4-29
... microfuge tube , and centrifuge at 12,000g for 5 minutes at 4 ° C in a microfuge . 3. Transfer about 1.2-1.3 ml of the supernatant to a fresh microfuge tube , and add 200 μl of a solution of 20 % polyethylene glycol ( PEG 8000 ) in 2.5 ...
... microfuge tube , and centrifuge at 12,000g for 5 minutes at 4 ° C in a microfuge . 3. Transfer about 1.2-1.3 ml of the supernatant to a fresh microfuge tube , and add 200 μl of a solution of 20 % polyethylene glycol ( PEG 8000 ) in 2.5 ...
Page 7-21
... tube just above the level of the remaining fluid . Place the bottom of the tube on a pad of Kimwipes , and carefully ... microfuge tube . Rinse the bottom of the ultracentrifuge tube with 25 μl of TE ( pH 7.6 ) and add to the microfuge ...
... tube just above the level of the remaining fluid . Place the bottom of the tube on a pad of Kimwipes , and carefully ... microfuge tube . Rinse the bottom of the ultracentrifuge tube with 25 μl of TE ( pH 7.6 ) and add to the microfuge ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml