Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xxxi
... Mutagenic Oligonucleotides 15.54 Hybridization of Oligonucleotides to the Template DNA and Primer Extension 15.57 ... Mutagenic Oligonucleotides 15.96 Treatment of Double - stranded DNA with Chemical Mutagens 15.105 Treatment of Single ...
... Mutagenic Oligonucleotides 15.54 Hybridization of Oligonucleotides to the Template DNA and Primer Extension 15.57 ... Mutagenic Oligonucleotides 15.96 Treatment of Double - stranded DNA with Chemical Mutagens 15.105 Treatment of Single ...
Page 1-49
... mutagenic activity of ethidium bromide in the Salmonella / microsome assay by approximately 3000 - fold . However , there are reports ( Lunn and Sansone 1987 ) of mutagenic activity in occasional batches of " blanks " treated with the ...
... mutagenic activity of ethidium bromide in the Salmonella / microsome assay by approximately 3000 - fold . However , there are reports ( Lunn and Sansone 1987 ) of mutagenic activity in occasional batches of " blanks " treated with the ...
Page 1-28
... mutagenic oligonucleotides , 15.96-15.104 using double - primer method , 15.55 , 15.58 , 15.63-15.65 to study proteins , 15.81-15.84 using chemical mutagens , 15.105 , 15.107 using sodium bisulfite , 15.106 MV1184 , E. coli strain ...
... mutagenic oligonucleotides , 15.96-15.104 using double - primer method , 15.55 , 15.58 , 15.63-15.65 to study proteins , 15.81-15.84 using chemical mutagens , 15.105 , 15.107 using sodium bisulfite , 15.106 MV1184 , E. coli strain ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml