Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xxx
... Mutants 15.14 GENERATION OF BIDIRECTIONAL SETS OF DELETION MUTANTS BY DIGESTION WITH NUCLEASE BAL 31 15.20 CLEAVAGE OF DOUBLE - STRANDED CLOSED CIRCULAR DNA WITH PANCREATIC DNAase I IN THE PRESENCE OF Mn ** 15.27 Linker - scanning ...
... Mutants 15.14 GENERATION OF BIDIRECTIONAL SETS OF DELETION MUTANTS BY DIGESTION WITH NUCLEASE BAL 31 15.20 CLEAVAGE OF DOUBLE - STRANDED CLOSED CIRCULAR DNA WITH PANCREATIC DNAase I IN THE PRESENCE OF Mn ** 15.27 Linker - scanning ...
Page 2-57
... mutants ( Kushner et al . 1974 ) . A bacterial gene that encodes exonuclease I , an enzyme that is part of the recF pathway . The sbcB mutation improves the growth of recB and recC mutants ( Kushner et al . 1971 ; Leach and Stahl 1983 ) ...
... mutants ( Kushner et al . 1974 ) . A bacterial gene that encodes exonuclease I , an enzyme that is part of the recF pathway . The sbcB mutation improves the growth of recB and recC mutants ( Kushner et al . 1971 ; Leach and Stahl 1983 ) ...
Page 4-18
... mutants of the helper that are resistant to interference ( see below ) ( Enea and Zinder 1982 ; Levinson et al . 1984 ) . The inclusion in the phagemid of sequences coding for the carboxy - terminal region of the gene IV protein appears ...
... mutants of the helper that are resistant to interference ( see below ) ( Enea and Zinder 1982 ; Levinson et al . 1984 ) . The inclusion in the phagemid of sequences coding for the carboxy - terminal region of the gene IV protein appears ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml