Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-58
... mutations Permissive for vectors carrying amber mutations Will modify but not restrict DNA Permissive for vectors carrying amber mutations Will modify but not restrict DNA Permissive for vectors carrying amber mutations Will modify but ...
... mutations Permissive for vectors carrying amber mutations Will modify but not restrict DNA Permissive for vectors carrying amber mutations Will modify but not restrict DNA Permissive for vectors carrying amber mutations Will modify but ...
Page 2-59
... mutations Used to screen recombinants made in vectors carrying a lacZ gene Nonpermissive for vectors carrying amber mutations Will not modify or restrict DNA recA strain Permissive for vectors carrying amber mutations Will not modify or ...
... mutations Used to screen recombinants made in vectors carrying a lacZ gene Nonpermissive for vectors carrying amber mutations Will not modify or restrict DNA recA strain Permissive for vectors carrying amber mutations Will not modify or ...
Page 2-95
... mutations in the A , D , or E gene ) . The lysogens also carry one or more of the following mutations : • cIts857 or imm434 clts , which specify a temperature - sensitive bacterio- phage à repressor molecule . This mutation causes ...
... mutations in the A , D , or E gene ) . The lysogens also carry one or more of the following mutations : • cIts857 or imm434 clts , which specify a temperature - sensitive bacterio- phage à repressor molecule . This mutation causes ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml