Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 75
Page 1-75
... obtained with E. coli strain LE392 or MC1061 and the plasmid pUC18 ( Dower et al . 1988 ) . In general , the authors claim that the efficiency of electrotransformation is 10 to 20 times higher than that obtained with maximally competent ...
... obtained with E. coli strain LE392 or MC1061 and the plasmid pUC18 ( Dower et al . 1988 ) . In general , the authors claim that the efficiency of electrotransformation is 10 to 20 times higher than that obtained with maximally competent ...
Page 1-76
... obtained and to store them in small aliquots in a dark , cool place . • The state of growth of the cells . For unknown reasons , the highest fre- quencies of transformation are obtained with cultures that have been grown directly from a ...
... obtained and to store them in small aliquots in a dark , cool place . • The state of growth of the cells . For unknown reasons , the highest fre- quencies of transformation are obtained with cultures that have been grown directly from a ...
Page 7-75
... obtained from duplicate samples of RNA , the following procedure is recommended : a . After step 2 , dissolve the RNA in 40-50 μl of water . Evaporate the sample in a rotary evaporator until it is just dry . b . Add 30 μl of ...
... obtained from duplicate samples of RNA , the following procedure is recommended : a . After step 2 , dissolve the RNA in 40-50 μl of water . Evaporate the sample in a rotary evaporator until it is just dry . b . Add 30 μl of ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml