Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 50
Page 183
Resuspend each pellet in 2 ml of ice - cold 0.1 m CaCl , ( or TFB , see Table 1.3
and notes to step 5 ) for each 50 ml of original culture . At this point , the cells can
be dispensed into aliquots that can be frozen at – 70 ° C ( for instructions , see ...
Resuspend each pellet in 2 ml of ice - cold 0.1 m CaCl , ( or TFB , see Table 1.3
and notes to step 5 ) for each 50 ml of original culture . At this point , the cells can
be dispensed into aliquots that can be frozen at – 70 ° C ( for instructions , see ...
Page 3-21
This " walking ” process is diagrammed in Figure 3.4 . The original cosmid is
digested with a restriction enzyme , such as HaeIII or Rsal , that cleaves foreign
DNA frequently but does not cleave within the bacteriophage T3 and T7
promoters .
This " walking ” process is diagrammed in Figure 3.4 . The original cosmid is
digested with a restriction enzyme , such as HaeIII or Rsal , that cleaves foreign
DNA frequently but does not cleave within the bacteriophage T3 and T7
promoters .
Page 3-35
In their original procedure , Ish - Horowicz and Burke did not select DNA
fragments of a given size to insert into the cosmid vector . Instead , highmolecular
- weight eukaryotic DNA was digested with Mbol until the average size of the
digestion ...
In their original procedure , Ish - Horowicz and Burke did not select DNA
fragments of a given size to insert into the cosmid vector . Instead , highmolecular
- weight eukaryotic DNA was digested with Mbol until the average size of the
digestion ...
What people are saying - Write a review
LibraryThing Review
User Review - iayork - LibraryThingthe BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review
LibraryThing Review
User Review - iayork - LibraryThingthe BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review
Other editions - View all
Common terms and phrases
activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies completely concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome growth Hindi host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations nucleic acids obtained original packaging particles pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes problem procedure promoter protein purified reaction recombinant Recover region remove replication restriction enzyme room temperature Sall segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume Xbal yield
References to this book
Bibliographic information
| Title | Molecular Cloning: A Laboratory Manual, Volume 1 Molecular Cloning: A Laboratory Manual, Joseph Sambrook |
| Authors | Joseph Sambrook, E. F. Fritsch, Tom Maniatis |
| Edition | 2 |
| Publisher | Cold Spring Harbor Laboratory, 1989 |
| Original from | the University of Michigan |
| Digitized | 23 Jul 2008 |
| ISBN | 0879693096, 9780879693091 |
| Length | 676 pages |
| Export Citation | BiBTeX EndNote RefMan |