Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-83
... original culture . At this point , the cells can be dispensed into aliquots that can be frozen at -70 ° C ( for instructions , see step 11b , iii , on page 1.80 ) . The cells maintain competency under these conditions , although the ...
... original culture . At this point , the cells can be dispensed into aliquots that can be frozen at -70 ° C ( for instructions , see step 11b , iii , on page 1.80 ) . The cells maintain competency under these conditions , although the ...
Page 3-21
... original cosmid is digested with a restriction enzyme , such as Hae III or Rsal , that cleaves foreign DNA frequently but does not cleave within the bac- teriophage T3 and T7 promoters . Among the digestion products will be two ...
... original cosmid is digested with a restriction enzyme , such as Hae III or Rsal , that cleaves foreign DNA frequently but does not cleave within the bac- teriophage T3 and T7 promoters . Among the digestion products will be two ...
Page 6-56
... original rinse buffer with an equal volume of fresh rinse buffer and continue incubation at 50 ° C for another hour . Caution : PMSF is extremely destructive to the mucous membranes of the respiratory tract , eyes , and skin . It may be ...
... original rinse buffer with an equal volume of fresh rinse buffer and continue incubation at 50 ° C for another hour . Caution : PMSF is extremely destructive to the mucous membranes of the respiratory tract , eyes , and skin . It may be ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml