Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-98
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Preparation of Packaging Extracts and Packaging of Bacteriophage λ DNA In Vitro Packaging extracts are available at reasonable cost from commercial sources . These work ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Preparation of Packaging Extracts and Packaging of Bacteriophage λ DNA In Vitro Packaging extracts are available at reasonable cost from commercial sources . These work ...
Page 2-104
... packaging reactions are stored in closed tubes at 4 ° C . Notes i . Each batch of extracts should be tested for packaging efficiency with a standardized preparation of intact bacteriophage › DNA . ii . These extracts exhibit a high ...
... packaging reactions are stored in closed tubes at 4 ° C . Notes i . Each batch of extracts should be tested for packaging efficiency with a standardized preparation of intact bacteriophage › DNA . ii . These extracts exhibit a high ...
Page 3-41
... packaging extracts ( Bates and Swift 1983 ) . Extracts used to package cosmids should be prepared using a buffer that contains spermidine but not putrescine . When putrescine is omitted from the packaging extract and the packaging ...
... packaging extracts ( Bates and Swift 1983 ) . Extracts used to package cosmids should be prepared using a buffer that contains spermidine but not putrescine . When putrescine is omitted from the packaging extract and the packaging ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml