Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-63
... plaques . For the same reason , it is advisable to pick plaques shortly after the bacterial lawn has grown up and the bacteriophage plaques have first appeared . Picking plaques early in their development also reduces the unwelcome ...
... plaques . For the same reason , it is advisable to pick plaques shortly after the bacterial lawn has grown up and the bacteriophage plaques have first appeared . Picking plaques early in their development also reduces the unwelcome ...
Page 2-108
... plaques by hybridization with 32P - labeled DNA probes ( Benton and Davis 1977 ) . Bacteriophages are plated at an appropriate density , and an imprint of the pattern of plaques is obtained by gently layering a nitrocellulose filter ...
... plaques by hybridization with 32P - labeled DNA probes ( Benton and Davis 1977 ) . Bacteriophages are plated at an appropriate density , and an imprint of the pattern of plaques is obtained by gently layering a nitrocellulose filter ...
Page 4-23
... plaques will be fully developed after 8-12 hours , and the blue color will intensify further if the plates are stored for a few hours at 4 ° C . Notes i . Colorless plaques ( formed by bacteriophages that are unable to accom- plish a ...
... plaques will be fully developed after 8-12 hours , and the blue color will intensify further if the plates are stored for a few hours at 4 ° C . Notes i . Colorless plaques ( formed by bacteriophages that are unable to accom- plish a ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml