Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-4
... plasmids is caused by inefficient binding of truncated RNA I to RNA II . In addition to controlling copy number , the regions of the plasmid encoding RNA I , RNA II , and the Rop protein also determine whether two different plasmids ...
... plasmids is caused by inefficient binding of truncated RNA I to RNA II . In addition to controlling copy number , the regions of the plasmid encoding RNA I , RNA II , and the Rop protein also determine whether two different plasmids ...
Page 1-5
... Plasmids like PSC101 are therefore under stringent replicative control and are present at only 5 copies per cell or less . Because stringently controlled replication requires ongoing expression of a plasmid - encoded protein , the copy ...
... Plasmids like PSC101 are therefore under stringent replicative control and are present at only 5 copies per cell or less . Because stringently controlled replication requires ongoing expression of a plasmid - encoded protein , the copy ...
Page 1-7
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Plasmid Vectors DEVELOPMENT OF PLASMID CLONING VECTORS The development of plasmid vectors has proceeded in three phases : 1. Selectable markers were introduced into plasmids ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Plasmid Vectors DEVELOPMENT OF PLASMID CLONING VECTORS The development of plasmid vectors has proceeded in three phases : 1. Selectable markers were introduced into plasmids ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml