Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xi
... Plasmid Vectors Essential Features of Plasmids 1.3 Replication and Incompatibility 1.3 Mobilization 1.5 Selectable Markers 1.5 Plasmid Vectors 1.7 DEVELOPMENT OF PLASMID CLONING VECTORS 1.7 Plasmid Vectors That Permit Histochemical ...
... Plasmid Vectors Essential Features of Plasmids 1.3 Replication and Incompatibility 1.3 Mobilization 1.5 Selectable Markers 1.5 Plasmid Vectors 1.7 DEVELOPMENT OF PLASMID CLONING VECTORS 1.7 Plasmid Vectors That Permit Histochemical ...
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A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Plasmid Vectors DEVELOPMENT OF PLASMID CLONING VECTORS The development of plasmid vectors has proceeded in three phases : 1. Selectable markers were introduced into plasmids ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Plasmid Vectors DEVELOPMENT OF PLASMID CLONING VECTORS The development of plasmid vectors has proceeded in three phases : 1. Selectable markers were introduced into plasmids ...
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... Plasmid Vectors In principle , cloning in plasmid vectors is very straightforward . The plasmid DNA is cleaved with a restriction enzyme and joined in vitro to foreign DNA . The resulting recombinant plasmids are then used to transform ...
... Plasmid Vectors In principle , cloning in plasmid vectors is very straightforward . The plasmid DNA is cleaved with a restriction enzyme and joined in vitro to foreign DNA . The resulting recombinant plasmids are then used to transform ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml