Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 50
Page 7-79
... PRIMER EXTENSION Primer extension is used to map and quantitate the 5 ' termini of RNA and to detect precursors and processing intermediates of mRNA . The test RNA is hybridized with an excess of a single - stranded DNA primer ( a ...
... PRIMER EXTENSION Primer extension is used to map and quantitate the 5 ' termini of RNA and to detect precursors and processing intermediates of mRNA . The test RNA is hybridized with an excess of a single - stranded DNA primer ( a ...
Page 7-80
... primer is not critical and can range from 75 to 150 or more nucleotides . However , the shorter the primer , the greater the difference in size between it and the extended product . 2. Mix 104-105 cpm of the DNA primer with 0.5-150 μg ...
... primer is not critical and can range from 75 to 150 or more nucleotides . However , the shorter the primer , the greater the difference in size between it and the extended product . 2. Mix 104-105 cpm of the DNA primer with 0.5-150 μg ...
Page 7-81
... primer are used per hybridization . However , because the number of artifactual bands increases as more primer is added to the hybridization reaction , it is advisable to carry out a series of pilot reactions that contain a constant ...
... primer are used per hybridization . However , because the number of artifactual bands increases as more primer is added to the hybridization reaction , it is advisable to carry out a series of pilot reactions that contain a constant ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
Copyright | |
96 other sections not shown
Other editions - View all
Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml