Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 89
Page xxiv
... PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 Removal of ...
... PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 Removal of ...
Page xxv
... Probes 10.29 Preparation of DNA Template 10.31 Synthesis of RNA In Vitro 10.32 Synthesis of RNA Probes Radiolabeled to High Specific Activity 10.34 Troubleshooting 10.36 SYNTHESIS OF cDNA PROBES 10.38 Identification of cDNA Clones 10.38 ...
... Probes 10.29 Preparation of DNA Template 10.31 Synthesis of RNA In Vitro 10.32 Synthesis of RNA Probes Radiolabeled to High Specific Activity 10.34 Troubleshooting 10.36 SYNTHESIS OF cDNA PROBES 10.38 Identification of cDNA Clones 10.38 ...
Page 7-71
... PROBES Radiolabeled RNA probes can be used in hybridization experiments in the same way as single - stranded DNA probes to detect and quantitate specific RNAs and to study gene and mRNA structure ( Lynn et al . 1983 ; Zinn et al . 1983 ...
... PROBES Radiolabeled RNA probes can be used in hybridization experiments in the same way as single - stranded DNA probes to detect and quantitate specific RNAs and to study gene and mRNA structure ( Lynn et al . 1983 ; Zinn et al . 1983 ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml