Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 90
Page 2-5
... proteins involved in DNA replication ) and terminate at tR2 . The leftward transcript codes for the N protein , whose action is essential for the next phase of infection . Delayed Early Transcription The N protein , by neutralizing the ...
... proteins involved in DNA replication ) and terminate at tR2 . The leftward transcript codes for the N protein , whose action is essential for the next phase of infection . Delayed Early Transcription The N protein , by neutralizing the ...
Page 2-7
... protein and proteolytic processing of other components , depends on a protein ( the groE gene product ) supplied by the host . The resulting structures are called preheads . Packaging the bacteriophage genome into preheads involves two ...
... protein and proteolytic processing of other components , depends on a protein ( the groE gene product ) supplied by the host . The resulting structures are called preheads . Packaging the bacteriophage genome into preheads involves two ...
Page 5-87
... Protein ( SSB ) ( E. coli or bacteriophage T4 - infected E. coli ) USES SSBS bind cooperatively to single - stranded DNA , but not to double - stranded DNA . Their usefulness ... Proteins 5 87 Single-stranded DNA-binding Protein (SSB) 5 87.
... Protein ( SSB ) ( E. coli or bacteriophage T4 - infected E. coli ) USES SSBS bind cooperatively to single - stranded DNA , but not to double - stranded DNA . Their usefulness ... Proteins 5 87 Single-stranded DNA-binding Protein (SSB) 5 87.
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
Copyright | |
96 other sections not shown
Other editions - View all
Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml