Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 4-7
... region , located between genes II and IV , into which seg- ments of foreign DNA may be inserted . This noncoding region is not dispensable , since it contains a cis - acting signal for the packaging and orientation of the DNA within ...
... region , located between genes II and IV , into which seg- ments of foreign DNA may be inserted . This noncoding region is not dispensable , since it contains a cis - acting signal for the packaging and orientation of the DNA within ...
Page 4-8
... region of M13mp1 usually eliminates a - complementation and gives rise to recombinants that form pale blue or colorless plaques ( Gronenborn and Messing 1978 ) . The development of this simple test to score for recombinants led to other ...
... region of M13mp1 usually eliminates a - complementation and gives rise to recombinants that form pale blue or colorless plaques ( Gronenborn and Messing 1978 ) . The development of this simple test to score for recombinants led to other ...
Page 4-18
... region version of gene II that works less well on its own MV1184 Vieira and Messing ( 1987 ) pBluescript f1 intergenic region than on that cloned in pUC118 / 119 M13K07 XL1 - Blue Short et al . ( 1988 ) Other more complicated phagemid ...
... region version of gene II that works less well on its own MV1184 Vieira and Messing ( 1987 ) pBluescript f1 intergenic region than on that cloned in pUC118 / 119 M13K07 XL1 - Blue Short et al . ( 1988 ) Other more complicated phagemid ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml