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The Nature of the Restriction Sites in the Plasmid Vector and the Foreign DNA
The variety of restriction sites in plasmid vectors is now extremely large , and it is
often possible to find a vector that carries exactly the same restriction sites as the
restriction enzyme cleavage site Digest with restriction enzyme A amp' ori \cos I It
Partially digest high-molecular- weight eukaryotic DNA with restriction enzyme
that generates termini compatible with restriction enzyme A 1 T amp' ori cos ...
1975 ) . Modification of Restriction Sites by DNA Methylation For many of the type
II restriction enzymes , a corresponding methylase has been isolated that
modifies the cognate recognition sequence and renders it resistant to cleavage .
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