Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 5-3
... Enzymes Restriction enzymes bind specifically to and cleave double - stranded DNA at specific sites within or adjacent to a particular sequence known as the recognition sequence . These enzymes have been classified into three groups ...
... Enzymes Restriction enzymes bind specifically to and cleave double - stranded DNA at specific sites within or adjacent to a particular sequence known as the recognition sequence . These enzymes have been classified into three groups ...
Page 5-16
... Restriction Sites by DNA Methylation For many of the type II restriction enzymes , a corresponding methylase has been isolated that modifies the cognate recognition sequence and renders it resistant to cleavage . These methylases , a ...
... Restriction Sites by DNA Methylation For many of the type II restriction enzymes , a corresponding methylase has been isolated that modifies the cognate recognition sequence and renders it resistant to cleavage . These methylases , a ...
Page 5-28
... ENZYMES Different manufacturers of restriction enzymes recommend significantly dif- ferent digestion conditions , even for the same ... Enzyme Activity 5.28 Enzymes Used in Molecular Cloning DIGESTING DNA WITH RESTRICTION ENZYMES 5 28.
... ENZYMES Different manufacturers of restriction enzymes recommend significantly dif- ferent digestion conditions , even for the same ... Enzyme Activity 5.28 Enzymes Used in Molecular Cloning DIGESTING DNA WITH RESTRICTION ENZYMES 5 28.
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml