Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 139
Filter the supernatant through four layers of cheesecloth into a 250 - ml centrifuge
bottle . Add 0.6 volume of isopropanol , mix well , and store the bottle for 10
minutes at room temperature . 7. Recover the nucleic acids by centrifugation at
5000 ...
Filter the supernatant through four layers of cheesecloth into a 250 - ml centrifuge
bottle . Add 0.6 volume of isopropanol , mix well , and store the bottle for 10
minutes at room temperature . 7. Recover the nucleic acids by centrifugation at
5000 ...
Page 140
Recover the precipitated nucleic acids by centrifugation at 10,000 rpm for 10
minutes at room temperature in a Sorvall SS34 rotor ( or its equivalent ) . 3.
Decant the supernatant carefully , and invert the open tube to allow the last drops
of ...
Recover the precipitated nucleic acids by centrifugation at 10,000 rpm for 10
minutes at room temperature in a Sorvall SS34 rotor ( or its equivalent ) . 3.
Decant the supernatant carefully , and invert the open tube to allow the last drops
of ...
Page 1-103
During the hybridization , the containers holding the filters should be tightly
closed to prevent the loss of fluid by evaporation . Alternatively , the probe may
be denatured by adding 0.1 volume of 3 N NaOH . After 5 minutes at room
temperature ...
During the hybridization , the containers holding the filters should be tightly
closed to prevent the loss of fluid by evaporation . Alternatively , the probe may
be denatured by adding 0.1 volume of 3 N NaOH . After 5 minutes at room
temperature ...
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LibraryThing Review
User Review - iayork - LibraryThingthe BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review
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Common terms and phrases
activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies completely concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome growth Hindi host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations nucleic acids obtained original packaging particles pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes problem procedure promoter protein purified reaction recombinant Recover region remove replication restriction enzyme room temperature Sall segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume Xbal yield