Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 55
Page 2-86
... sample several times with 2 - butanol ( see Appendix E ) to reduce the volume to less than 3 ml . Alternatively , if the pooled sample is small , the DNA can be precipitated with ethanol without prior dialysis by diluting the sample ...
... sample several times with 2 - butanol ( see Appendix E ) to reduce the volume to less than 3 ml . Alternatively , if the pooled sample is small , the DNA can be precipitated with ethanol without prior dialysis by diluting the sample ...
Page 6-43
... sample tends to dribble out of the loading device after the tip is immersed in the electrophoresis buffer . Usually , about 3-5 μl of DNA sample are loaded per well ( 0.5 cm × 0.3 cm × 1 mm ) . Raise the loading device as the sample is ...
... sample tends to dribble out of the loading device after the tip is immersed in the electrophoresis buffer . Usually , about 3-5 μl of DNA sample are loaded per well ( 0.5 cm × 0.3 cm × 1 mm ) . Raise the loading device as the sample is ...
Page 7-53
... sample of the RNA preparation onto dry nitrocellulose , which was then dried , hybrid- ized with a specific 32P ... samples and to deposit the nucleic acids onto the nitrocellulose in a fixed pattern that allows the results to be ...
... sample of the RNA preparation onto dry nitrocellulose , which was then dried , hybrid- ized with a specific 32P ... samples and to deposit the nucleic acids onto the nitrocellulose in a fixed pattern that allows the results to be ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
Copyright | |
96 other sections not shown
Other editions - View all
Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml