Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 6-43
... sample out of the well . The same device can be used to load many samples , provided it is thoroughly washed between each loading . However , it is important not to take too long to complete loading the gel ; otherwise , the samples ...
... sample out of the well . The same device can be used to load many samples , provided it is thoroughly washed between each loading . However , it is important not to take too long to complete loading the gel ; otherwise , the samples ...
Page 7-44
... samples before electrophoresis ( Fourney et al . 1988 ) . Although this solves the problem of staining RNA in the gel after electrophoresis , it can reduce the efficiency of northern hybridization , especially when the samples contain ...
... samples before electrophoresis ( Fourney et al . 1988 ) . Although this solves the problem of staining RNA in the gel after electrophoresis , it can reduce the efficiency of northern hybridization , especially when the samples contain ...
Page 7-53
... samples are applied . The manifold fits onto a suction platform containing a sheet of nitrocellulose onto which the samples are deposited . These manifolds are available commercially ( e.g. , Minifold II , Schleicher and Schuell ) . 2 ...
... samples are applied . The manifold fits onto a suction platform containing a sheet of nitrocellulose onto which the samples are deposited . These manifolds are available commercially ( e.g. , Minifold II , Schleicher and Schuell ) . 2 ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml