Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 32
Page 1-8
... segment of foreign DNA . In most cases , insertion of the foreign sequences inactivates the marker . Transformants containing recombinant plasmids can therefore be distinguished from those carrying the parental vector by virtue of their ...
... segment of foreign DNA . In most cases , insertion of the foreign sequences inactivates the marker . Transformants containing recombinant plasmids can therefore be distinguished from those carrying the parental vector by virtue of their ...
Page 1-54
... Foreign DNA to Plasmid Vectors Termini carried by fragment of foreign DNA Blunt - ended Different protruding termini ... segment of foreign DNA that contains cohesive termini compatible with those generated by BamHI and HindIII . The ...
... Foreign DNA to Plasmid Vectors Termini carried by fragment of foreign DNA Blunt - ended Different protruding termini ... segment of foreign DNA that contains cohesive termini compatible with those generated by BamHI and HindIII . The ...
Page 4-7
... foreign DNA segments within this intergenic region can severely affect the replication of the bacteriophage genome ( Dotto and Zinder 1984a ) . Fortunately , filamentous bacteriophages carrying foreign DNA se- quences in the intergenic ...
... foreign DNA segments within this intergenic region can severely affect the replication of the bacteriophage genome ( Dotto and Zinder 1984a ) . Fortunately , filamentous bacteriophages carrying foreign DNA se- quences in the intergenic ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
Copyright | |
96 other sections not shown
Other editions - View all
Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml