Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 4-20
... single - stranded copies of fragments of DNA that have already been cloned in another type of vector ( a plasmid or phagemid ) . Single - stranded DNA can be obtained in this way with such ease and in such high yield that filamentous ...
... single - stranded copies of fragments of DNA that have already been cloned in another type of vector ( a plasmid or phagemid ) . Single - stranded DNA can be obtained in this way with such ease and in such high yield that filamentous ...
Page 4-52
... single - stranded DNA in vitro introduces new restriction endo- nuclease cleavage sites . Nature 272 : 375 . Herrmann , R. , K. Neugebauer , E. Pirkl , H. Zentgraf , and H. Schaller . 1980 . Conversion of bacteriophage fd into an ...
... single - stranded DNA in vitro introduces new restriction endo- nuclease cleavage sites . Nature 272 : 375 . Herrmann , R. , K. Neugebauer , E. Pirkl , H. Zentgraf , and H. Schaller . 1980 . Conversion of bacteriophage fd into an ...
Page 7-66
... Single - stranded DNA Probes Single - stranded DNA probes are prepared by one of two methods : · Separation of the strands of a fragment of double - stranded DNA • De novo synthesis of a strand from a single - stranded template Strand ...
... Single - stranded DNA Probes Single - stranded DNA probes are prepared by one of two methods : · Separation of the strands of a fragment of double - stranded DNA • De novo synthesis of a strand from a single - stranded template Strand ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml