Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 142
Measure the volume of the DNA solution . For every milliliter , add exactly 1g of
solid CsCl . Warm the solution to 30 ° C to facilitate the dissolution of the salt . Mix
the solution gently until the salt is dissolved . 2. Add 0.8 ml of a solution of ...
Measure the volume of the DNA solution . For every milliliter , add exactly 1g of
solid CsCl . Warm the solution to 30 ° C to facilitate the dissolution of the salt . Mix
the solution gently until the salt is dissolved . 2. Add 0.8 ml of a solution of ...
Page 144
DISCONTINUOUS GRADIENTS In this method , solutions containing different
concentrations of CsCl are layered into the centrifuge tube . ... Prepare a solution
of CsCl ( p = 1.47 g / ml ) by adding 125 g of CsCl to 167 ml of TE ( pH 8.0 ) . 2.
DISCONTINUOUS GRADIENTS In this method , solutions containing different
concentrations of CsCl are layered into the centrifuge tube . ... Prepare a solution
of CsCl ( p = 1.47 g / ml ) by adding 125 g of CsCl to 167 ml of TE ( pH 8.0 ) . 2.
Page 178
The pH of the solution is then adjusted to 6.3 with 5 M KOH , and pure H , O is
then added to bring the final volume to 100 ml . The solution is sterilized by
filtration through a disposable Nalgene filter ( 0.45 - micron pore size ) , divided
into 10 ...
The pH of the solution is then adjusted to 6.3 with 5 M KOH , and pure H , O is
then added to bring the final volume to 100 ml . The solution is sterilized by
filtration through a disposable Nalgene filter ( 0.45 - micron pore size ) , divided
into 10 ...
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User Review - iayork - LibraryThingthe BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review
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Common terms and phrases
activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies completely concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome growth Hindi host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations nucleic acids obtained original packaging particles pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes problem procedure promoter protein purified reaction recombinant Recover region remove replication restriction enzyme room temperature Sall segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume Xbal yield
References to this book
Bibliographic information
| Title | Molecular Cloning: A Laboratory Manual, Volume 1 Molecular Cloning: A Laboratory Manual, Joseph Sambrook |
| Authors | Joseph Sambrook, E. F. Fritsch, Tom Maniatis |
| Edition | 2 |
| Publisher | Cold Spring Harbor Laboratory, 1989 |
| Original from | the University of Michigan |
| Digitized | 23 Jul 2008 |
| ISBN | 0879693096, 9780879693091 |
| Length | 676 pages |
| Export Citation | BiBTeX EndNote RefMan |