Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-42
... solution to 30 ° C to facilitate the dissolution of the salt . Mix the solution gently until the salt is dissolved . 2. Add 0.8 ml of a solution of ethidium bromide ( 10 mg / ml in water ) for every 10 ml of the DNA / CsCl solution ...
... solution to 30 ° C to facilitate the dissolution of the salt . Mix the solution gently until the salt is dissolved . 2. Add 0.8 ml of a solution of ethidium bromide ( 10 mg / ml in water ) for every 10 ml of the DNA / CsCl solution ...
Page 1-44
... solution to a Beckman Quick - Seal centrifuge tube ( or its equivalent ) . Put this aside until needed in step 8 . 3. If necessary , adjust the volume of the solution of plasmid DNA to exactly 3 ml with TE ( pH 8.0 ) . 4. Add 8.4 g of ...
... solution to a Beckman Quick - Seal centrifuge tube ( or its equivalent ) . Put this aside until needed in step 8 . 3. If necessary , adjust the volume of the solution of plasmid DNA to exactly 3 ml with TE ( pH 8.0 ) . 4. Add 8.4 g of ...
Page 1-78
... solution to 1 liter with pure H2O . Sterilize the solution by filtration through a disposable Nalgene filter . Dispense the solution into 40 - ml aliquots , and store the aliquots in tissue - culture flasks ( Corning 25100 or equivalent ) ...
... solution to 1 liter with pure H2O . Sterilize the solution by filtration through a disposable Nalgene filter . Dispense the solution into 40 - ml aliquots , and store the aliquots in tissue - culture flasks ( Corning 25100 or equivalent ) ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml