Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 82
Page 5-84
... specific for apurinic DNA , an RNAase H activity ( Rogers and Weiss 1980 ) , and a 3 ' phosphatase activity , which removes 3 ' - phosphate termini but does not cleave internal phosphodiester bonds . The exonuclease will not degrade ...
... specific for apurinic DNA , an RNAase H activity ( Rogers and Weiss 1980 ) , and a 3 ' phosphatase activity , which removes 3 ' - phosphate termini but does not cleave internal phosphodiester bonds . The exonuclease will not degrade ...
Page 7-53
... specific gene expression in specific tissues or cultured cells . Recently , this technique has been improved in two ways : 1. Filtration manifolds have been designed to accept a large number of samples and to deposit the nucleic acids ...
... specific gene expression in specific tissues or cultured cells . Recently , this technique has been improved in two ways : 1. Filtration manifolds have been designed to accept a large number of samples and to deposit the nucleic acids ...
Page 7-84
... specific RNAS or specific fragments of DNA by frac- tionation in gels and transfer to diazobenzyloxymethyl paper . Methods Enzymol . 68 : 220 . Aviv , H. and P. Leder . 1972. Purification of biologically active globin messenger RNA by ...
... specific RNAS or specific fragments of DNA by frac- tionation in gels and transfer to diazobenzyloxymethyl paper . Methods Enzymol . 68 : 220 . Aviv , H. and P. Leder . 1972. Purification of biologically active globin messenger RNA by ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
Copyright | |
96 other sections not shown
Other editions - View all
Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml