Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-55
... strains of E. coli that have been designed to serve as specific hosts . Table 2.2 provides descriptions of some of the genetic markers found in these strains . The strains themselves are listed in Table 2.3 and are more fully described ...
... strains of E. coli that have been designed to serve as specific hosts . Table 2.2 provides descriptions of some of the genetic markers found in these strains . The strains themselves are listed in Table 2.3 and are more fully described ...
Page 2-57
... Strains supE supF hflA recA recB recC sbcA sbc B recD lacl lacz hsdR hsdS hsdM mcrB lon rpsL dam Bacterial genes encoding suppressor tRNAs for amber mutations . supE inserts a glutamine residue at the codon UAG ; supF inserts a tyrosine ...
... Strains supE supF hflA recA recB recC sbcA sbc B recD lacl lacz hsdR hsdS hsdM mcrB lon rpsL dam Bacterial genes encoding suppressor tRNAs for amber mutations . supE inserts a glutamine residue at the codon UAG ; supF inserts a tyrosine ...
Page 5-15
... Strains of E. coli Most strains of E. coli contain two enzymes that methylate DNA - the dam methylase and the dcm methylase . dam METHYLASE This enzyme introduces methyl groups at the N ° position of adenine in the sequence 5 ′ GATC 3 ...
... Strains of E. coli Most strains of E. coli contain two enzymes that methylate DNA - the dam methylase and the dcm methylase . dam METHYLASE This enzyme introduces methyl groups at the N ° position of adenine in the sequence 5 ′ GATC 3 ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml