Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xxii
... cDNA Libraries Strategies for cDNA Cloning 8.3 PREPARATION OF mRNA FOR CDNA CLONING 8.3 Source of the mRNA 8.3 ... STRAND OF cDNA 8.11 SYNTHESIS OF THE SECOND STRAND OF cDNA 8.14 Self - priming 8.14 Replacement Synthesis of the Second ...
... cDNA Libraries Strategies for cDNA Cloning 8.3 PREPARATION OF mRNA FOR CDNA CLONING 8.3 Source of the mRNA 8.3 ... STRAND OF cDNA 8.11 SYNTHESIS OF THE SECOND STRAND OF cDNA 8.14 Self - priming 8.14 Replacement Synthesis of the Second ...
Page xxiii
... Strand of cDNA 8.64 Methylation of cDNA 8.66 Ligation to Synthetic Phosphorylated Linkers 8.68 Size Selection of cDNA 8.70 Ligation to Bacteriophage λ Arms 8.73 Analysis of cDNA Inserts 8.76 Generation of a Complete cDNA Library 8.77 ...
... Strand of cDNA 8.64 Methylation of cDNA 8.66 Ligation to Synthetic Phosphorylated Linkers 8.68 Size Selection of cDNA 8.70 Ligation to Bacteriophage λ Arms 8.73 Analysis of cDNA Inserts 8.76 Generation of a Complete cDNA Library 8.77 ...
Page 5-53
... stranded cDNA ( see Chapter 8 ) . If necessary , both self - primed and exogenously primed second - strand Α 5 ' synthesis can be inhibited by including actinomycin Enzymes Used in Molecular Cloning 5.53 SYNTHESIS OF THE FIRST STRAND OF ...
... stranded cDNA ( see Chapter 8 ) . If necessary , both self - primed and exogenously primed second - strand Α 5 ' synthesis can be inhibited by including actinomycin Enzymes Used in Molecular Cloning 5.53 SYNTHESIS OF THE FIRST STRAND OF ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml