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Filter the supernatant through four layers of cheesecloth into a 250 - ml centrifuge
bottle . Add 0.6 volume of isopropanol , mix well , and store the bottle for 10
minutes at room temperature . 7. Recover the nucleic acids by centrifugation at
Transfer the supernatant to a fresh 30 - ml Corex tube . Add an equal volume of
isopropanol . Mix well . Recover the precipitated nucleic acids by centrifugation at
10,000 rpm for 10 minutes at room temperature in a Sorvall SS34 rotor ( or its ...
Transfer the supernatant set aside in step 4 ( page 4.31 ) to a 500 - ml beaker
containing a magnetic stirring bar . 2. Add 10 g of polyethylene glycol ( PEG 8000
) and 7.5 g of NaCl to the supernatant , and stir for 30 minutes to 1 hour at room ...
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the BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review