Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-64
... termini in the solution . For double- stranded DNA with self - complementary cohesive termini , -3 i = 2 N ̧M × 10 - 3 ends / ml O where N is Avogadro's number and M is the molar concentration of the DNA . Theoretically , when j = i ...
... termini in the solution . For double- stranded DNA with self - complementary cohesive termini , -3 i = 2 N ̧M × 10 - 3 ends / ml O where N is Avogadro's number and M is the molar concentration of the DNA . Theoretically , when j = i ...
Page 2-90
... termini of the vector be reannealed and ligated before treatment with phosphatase ; otherwise , concatemerization will be inhibited and the packaging efficiency of the DNA will be reduced signifi- cantly . 1. Ligate the cohesive termini ...
... termini of the vector be reannealed and ligated before treatment with phosphatase ; otherwise , concatemerization will be inhibited and the packaging efficiency of the DNA will be reduced signifi- cantly . 1. Ligate the cohesive termini ...
Page 5-57
... termini of DNA if modified nucleotides ( e.g. , ddNTPs or cordycepin tri- phosphate ) are used as substrates . Homopolymers of rNTPs can also be synthesized at the 3 ' termini of DNA molecules in the presence of Co ** ( for references ...
... termini of DNA if modified nucleotides ( e.g. , ddNTPs or cordycepin tri- phosphate ) are used as substrates . Homopolymers of rNTPs can also be synthesized at the 3 ' termini of DNA molecules in the presence of Co ** ( for references ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml