Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xxiv
... TRANSFER OF DNA FROM AGAROSE GELS TO SOLID SUPPORTS 9.34 Transfer of DNA to Nitrocellulose Filters 9.38 CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 9.38 SIMULTANEOUS TRANSFER OF DNA FROM A SINGLE AGAROSE GEL TO TWO ...
... TRANSFER OF DNA FROM AGAROSE GELS TO SOLID SUPPORTS 9.34 Transfer of DNA to Nitrocellulose Filters 9.38 CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 9.38 SIMULTANEOUS TRANSFER OF DNA FROM A SINGLE AGAROSE GEL TO TWO ...
Page 7-46
... transfer , or electroblotting ( see Chapter 9 , pages 9.34-9.37 , for a discussion of the relative merits of these techniques ) . Capillary elution is carried out as described below ; vacuum transfer and electroblotting should be ...
... transfer , or electroblotting ( see Chapter 9 , pages 9.34-9.37 , for a discussion of the relative merits of these techniques ) . Capillary elution is carried out as described below ; vacuum transfer and electroblotting should be ...
Page 7-49
... transfer RNA from gels to nylon membranes are similar to those used for transfer to nitrocellulose filters and include vacuum trans- fer , electroblotting , or capillary elution ( see Chapter 9 , pages 9.34-9.37 , for a discussion of ...
... transfer RNA from gels to nylon membranes are similar to those used for transfer to nitrocellulose filters and include vacuum trans- fer , electroblotting , or capillary elution ( see Chapter 9 , pages 9.34-9.37 , for a discussion of ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml