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Place the tube in a boiling - water bath for exactly 40 seconds . 4. Centrifuge the
bacterial lysate at 12,000g for 10 minutes at room temperature in a microfuge . 5.
Remove the pellet of bacterial debris from the microfuge tube with a sterile ...
DISCONTINUOUS GRADIENTS In this method , solutions containing different
concentrations of CsCl are layered into the centrifuge tube . This speeds the
formation of the CsCl gradient and allows the centrifugation time to be reduced to
Using a red - hot razor blade held in a hemostat , cut off the bottom of the tube
just above the level of the remaining fluid . Place the bottom of the tube on a pad
of Kimwipes , and carefully withdraw the fluid with an automatic pipettor . Invert
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LibraryThing ReviewUser Review - iayork - LibraryThing
the BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review