Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 92
Page 1-53
... vectors is very straightforward . The plasmid DNA is cleaved with a restriction enzyme and joined in vitro to foreign DNA . The resulting recombinant plasmids are then used to transform bacteria . In practice , however , the plasmid vector ...
... vectors is very straightforward . The plasmid DNA is cleaved with a restriction enzyme and joined in vitro to foreign DNA . The resulting recombinant plasmids are then used to transform bacteria . In practice , however , the plasmid vector ...
Page 1-66
... vector DNA : foreign DNA . For most routine cloning purposes , this is unnecessary . The curves in Figures 1.9 , 1.10 , and 1.11 show that reasonable numbers of useful recombinants will be obtained when the ratio of plasmid DNA : foreign ...
... vector DNA : foreign DNA . For most routine cloning purposes , this is unnecessary . The curves in Figures 1.9 , 1.10 , and 1.11 show that reasonable numbers of useful recombinants will be obtained when the ratio of plasmid DNA : foreign ...
Page 2-82
... DNA sequences propagated in vectors with larger capacity ( e.g. , cosmids ) to construction of complex cDNA or genomic DNA libraries . Cloning in bacteriophage λ ... Vectors Cloning in Bacteriophage λ 2 82 PREPARATION OF VECTOR DNA 2 82.
... DNA sequences propagated in vectors with larger capacity ( e.g. , cosmids ) to construction of complex cDNA or genomic DNA libraries . Cloning in bacteriophage λ ... Vectors Cloning in Bacteriophage λ 2 82 PREPARATION OF VECTOR DNA 2 82.
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml