Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-49
... volume of fresh 5 % hypophosphorous acid and 0.12 volume of fresh 0.5 M sodium nitrite . Mix carefully . Important : Check that the pH of the solution is < 3.0 . Hypophosphorous acid is usually supplied as a 50 % solution , which is ...
... volume of fresh 5 % hypophosphorous acid and 0.12 volume of fresh 0.5 M sodium nitrite . Mix carefully . Important : Check that the pH of the solution is < 3.0 . Hypophosphorous acid is usually supplied as a 50 % solution , which is ...
Page 6-31
... volumes of TE ( pH 7.6 ) . This step helps to prevent precipitation of oligosaccharides . Add 0.05 volume of 5 M NaCl followed by 2 volumes of ethanol . Incubate the tube at 0 ° C for 15 minutes and then collect the precipitate by ...
... volumes of TE ( pH 7.6 ) . This step helps to prevent precipitation of oligosaccharides . Add 0.05 volume of 5 M NaCl followed by 2 volumes of ethanol . Incubate the tube at 0 ° C for 15 minutes and then collect the precipitate by ...
Page 7-21
... Volume of homogenate 3.3 ml 10.0 ml 26.0 ml Volume of TE ( pH 7.6 ) / 0.1 % SDS 100 μl 300 με 1.0 ml If the RNA is difficult to dissolve , freeze and thaw the sample twice and then heat it to 45 ° C . This usually breaks up the pellets ...
... Volume of homogenate 3.3 ml 10.0 ml 26.0 ml Volume of TE ( pH 7.6 ) / 0.1 % SDS 100 μl 300 με 1.0 ml If the RNA is difficult to dissolve , freeze and thaw the sample twice and then heat it to 45 ° C . This usually breaks up the pellets ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml