Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 55
Page 1-103
Agitate the filters gently , and turn them over at least once during washing . After 5
minutes , transfer the filters to a fresh batch of wash solution and continue to
agitate them gently . Repeat the washing procedure twice more . At no stage
during ...
Agitate the filters gently , and turn them over at least once during washing . After 5
minutes , transfer the filters to a fresh batch of wash solution and continue to
agitate them gently . Repeat the washing procedure twice more . At no stage
during ...
Page 2-116
Agitate the filters gently , and turn them over at least once during washing . After 5
minutes , transfer the filters to a fresh batch of wash solution and continue to
agitate them gently . Repeat the washing procedure twice more . At no stage
during ...
Agitate the filters gently , and turn them over at least once during washing . After 5
minutes , transfer the filters to a fresh batch of wash solution and continue to
agitate them gently . Repeat the washing procedure twice more . At no stage
during ...
Page 6-53
Procedures to lyse mammalian cells and yeasts and to digest the liberated DNA
in situ are described below . ISOLATION OF INTACT DNA FROM MAMMALIAN
CELLS 1. Prepare cells or tissue samples as follows : • Cultured cells . Wash
cells ...
Procedures to lyse mammalian cells and yeasts and to digest the liberated DNA
in situ are described below . ISOLATION OF INTACT DNA FROM MAMMALIAN
CELLS 1. Prepare cells or tissue samples as follows : • Cultured cells . Wash
cells ...
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User Review - iayork - LibraryThingthe BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review
LibraryThing Review
User Review - iayork - LibraryThingthe BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review
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Common terms and phrases
activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies completely concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome growth Hindi host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations nucleic acids obtained original packaging particles pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes problem procedure promoter protein purified reaction recombinant Recover region remove replication restriction enzyme room temperature Sall segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume Xbal yield