Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-3
... fragments were fractionated by density gradient centrifugation or prepara ... DNA : • The ability to " walk " along the eukaryotic chromosome in a way that ... DNA Libraries Bacteriophage λ Analysis and Cloning of Eukaryotic Genomic DNA 9.3.
... fragments were fractionated by density gradient centrifugation or prepara ... DNA : • The ability to " walk " along the eukaryotic chromosome in a way that ... DNA Libraries Bacteriophage λ Analysis and Cloning of Eukaryotic Genomic DNA 9.3.
Page 10-52
... DNA with the desired restriction enzyme in 25 μl of the appropriate restriction enzyme buffer . 2. Add 2-10 μμCi ( sp . act . 400-3000 Ci / mmole ) of the appropriate [ a- 32P ] dNTP . 3. Add 1 unit of the Klenow fragment of E. coli DNA ...
... DNA with the desired restriction enzyme in 25 μl of the appropriate restriction enzyme buffer . 2. Add 2-10 μμCi ( sp . act . 400-3000 Ci / mmole ) of the appropriate [ a- 32P ] dNTP . 3. Add 1 unit of the Klenow fragment of E. coli DNA ...
Page 15-92
... DNA fragment carrying the kan ' gene has been inserted into the amp ' gene rather than into the target sequence . 9. Pick 36 independent transformants , and establish small - scale ( 2 - ml ) cultures in LB medium containing ampicillin ...
... DNA fragment carrying the kan ' gene has been inserted into the amp ' gene rather than into the target sequence . 9. Pick 36 independent transformants , and establish small - scale ( 2 - ml ) cultures in LB medium containing ampicillin ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector