Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 13-6
... sequencing technique ( Sanger and Coulson 1975 ) , which first described ( 1 ) the use of a specific primer for extension by DNA polymerase , ( 2 ) base - specific chain termination , and ( 3 ) the use of polyacrylamide gels to ...
... sequencing technique ( Sanger and Coulson 1975 ) , which first described ( 1 ) the use of a specific primer for extension by DNA polymerase , ( 2 ) base - specific chain termination , and ( 3 ) the use of polyacrylamide gels to ...
Page 13-14
A Laboratory Manual Joseph Sambrook, Tom Maniatis. SEQUENCING STRATEGIES Before beginning to sequence , it is important to develop an ... DNA Sequencing SEQUENCING STRATEGIES 13 14 Confirmatory Sequencing 13 14 De Novo Sequencing 13 14.
A Laboratory Manual Joseph Sambrook, Tom Maniatis. SEQUENCING STRATEGIES Before beginning to sequence , it is important to develop an ... DNA Sequencing SEQUENCING STRATEGIES 13 14 Confirmatory Sequencing 13 14 De Novo Sequencing 13 14.
Page 13-18
... sequencing project relies heavily on computer programs to sort and order the primary sequence data ( Staden 1986 ) . Access to adequate computing ... DNA . 13.18 DNA Sequencing FACTORS AFFECTING THE CHOICE BETWEEN RANDOM AND DIRECTED 18.
... sequencing project relies heavily on computer programs to sort and order the primary sequence data ( Staden 1986 ) . Access to adequate computing ... DNA . 13.18 DNA Sequencing FACTORS AFFECTING THE CHOICE BETWEEN RANDOM AND DIRECTED 18.
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector