Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 13-28
A Laboratory Manual Joseph Sambrook, Tom Maniatis. DIGESTION WITH DNAase I IN THE PRESENCE OF MANGANESE IONS Pancreatic DNAase I normally introduces single - strand nicks into double- stranded DNA . In the presence of Mn ++ , however ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. DIGESTION WITH DNAase I IN THE PRESENCE OF MANGANESE IONS Pancreatic DNAase I normally introduces single - strand nicks into double- stranded DNA . In the presence of Mn ++ , however ...
Page 15-5
... DNAase I. Closed circular plasmid DNA is digested with limited amounts of DNAase I in the presence of a transition metal ion such as Mn ++ or Co ** ( Heffron et al . 1978 ) . Under these conditions , DNAase I attacks DNA randomly ...
... DNAase I. Closed circular plasmid DNA is digested with limited amounts of DNAase I in the presence of a transition metal ion such as Mn ++ or Co ** ( Heffron et al . 1978 ) . Under these conditions , DNAase I attacks DNA randomly ...
Page 15-27
... DNAase I IN THE PRESENCE OF Mn ++ The following protocol is an adaptation of methods described by Anderson ( 1981 ) and Labeit et al . ( 1987 ) . The principle of the method is shown in Figure 15.3 . In brief , a double - stranded ...
... DNAase I IN THE PRESENCE OF Mn ++ The following protocol is an adaptation of methods described by Anderson ( 1981 ) and Labeit et al . ( 1987 ) . The principle of the method is shown in Figure 15.3 . In brief , a double - stranded ...
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agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector