Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-16
... ( pH 8.0 ) 0.1 м EDTA ( pH 8.0 ) 20 μg / ml pancreatic RNAase 0.5 % SDS It is important that the cells be well - dispersed over the inner surface of the Erlen- meyer flask when the extraction buffer is added . This minimizes the formation ...
... ( pH 8.0 ) 0.1 м EDTA ( pH 8.0 ) 20 μg / ml pancreatic RNAase 0.5 % SDS It is important that the cells be well - dispersed over the inner surface of the Erlen- meyer flask when the extraction buffer is added . This minimizes the formation ...
Page 9-20
... ( pH 8.0 ) , 10 mм EDTA ( pH 8.0 ) and six times against 4 liters of 10 mM NaCl , 10 mM Tris Cl ( pH 8.0 ) , 0.5 mM EDTA ( pH 8.0 ) . . · 4. Measure the absorbance of the DNA at 260 nm and 280 nm . The ratio of A260 to A280 should be ...
... ( pH 8.0 ) , 10 mм EDTA ( pH 8.0 ) and six times against 4 liters of 10 mM NaCl , 10 mM Tris Cl ( pH 8.0 ) , 0.5 mM EDTA ( pH 8.0 ) . . · 4. Measure the absorbance of the DNA at 260 nm and 280 nm . The ratio of A260 to A280 should be ...
Page 13-84
... acid ( 17.4 M ) . 99 % ( Aldrich ; Gold label 99 % , catalog no . 018 63-009 ) . This solution is made by mixing 1 volume of dimethyl sulfate with 9 volumes of ethanol . 50 mM sodium cacodylate ( pH 7.0 ) 1 mM EDTA ( pH 8.0 ) 1.5 M sodium ...
... acid ( 17.4 M ) . 99 % ( Aldrich ; Gold label 99 % , catalog no . 018 63-009 ) . This solution is made by mixing 1 volume of dimethyl sulfate with 9 volumes of ethanol . 50 mM sodium cacodylate ( pH 7.0 ) 1 mM EDTA ( pH 8.0 ) 1.5 M sodium ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector