Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 89
Page 8-85
... Escherichia coli . J. Biol . Chem . 260 : 9326 . Rougeon , F. and B. Mach . 1976. Stepwise biosynthesis in vitro of globin genes from globin mRNA by DNA polymer- ase of avian myeloblastosis virus . Proc . Natl . Acad . Sci . 73 : 3418 ...
... Escherichia coli . J. Biol . Chem . 260 : 9326 . Rougeon , F. and B. Mach . 1976. Stepwise biosynthesis in vitro of globin genes from globin mRNA by DNA polymer- ase of avian myeloblastosis virus . Proc . Natl . Acad . Sci . 73 : 3418 ...
Page 10-69
... Escherichia coli . IV . An exonuclease activity pres- ent in purified preparations of deoxy- ribonucleic acid polymerase . J. Biol . Chem . 239 : 233 . Lerman , L.S. 1971. A transition to a com- pact form of DNA in polymer solutions ...
... Escherichia coli . IV . An exonuclease activity pres- ent in purified preparations of deoxy- ribonucleic acid polymerase . J. Biol . Chem . 239 : 233 . Lerman , L.S. 1971. A transition to a com- pact form of DNA in polymer solutions ...
Page 12-41
... Escherichia coli . Gene 25 : 167 . Bagdasarian , M.M. , E. Amann , R. Lurz , B. Rückert , and M. Bagdasarian . 1983 . Activity of the hybrid trp - lac ( tac ) pro- moter of Escherichia coli in Pseudo- monas putida . Construction of ...
... Escherichia coli . Gene 25 : 167 . Bagdasarian , M.M. , E. Amann , R. Lurz , B. Rückert , and M. Bagdasarian . 1983 . Activity of the hybrid trp - lac ( tac ) pro- moter of Escherichia coli in Pseudo- monas putida . Construction of ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector